. 2016 Dec 3;13(2):191–201. doi: 10.1007/s11302-016-9549-9

Table 2.

The (apparent) association and dissociation rate constants of LUF7445, LUF6632, and ZM241385 determined in competition association assays with [³H]-ZM241385 binding to HEK293-hA_2AAR membranes

Compound	k _on (nM⁻¹ min⁻¹)^a	k _off (min⁻¹)^a	RT (min)
LUF7445^b	(0.0059 ± 0.00049)	(1.37 ± 0.68 × 10⁻¹¹)	(2.86 ± 0.87 × 10¹¹)
LUF6632	1.53 ± 0.083	0.15 ± 0.021	6.80 ± 0.97
ZM241385	1.72 ± 0.36	1.04 ± 0.13	0.96 ± 0.12

^aAssociation (k _on) and dissociation (k _off) rate constants were determined by competition association assay at 25 °C; all these values were determined by analyzing the data in the mathematical model described by Motulsky and Mahan [27]

^bFor LUF7445, no equilibrium is reached between receptors and ligand; hence, the Motulsky/Mahan mathematical model [27] for the competition association assay is not valid. The values obtained are therefore considered to provide qualitative insight only, and are in brackets