Fig. 3.

Effect of prasugrel alone or in combination with lithium on the protein abundances of NKCC2 and NCC in the renal cortex and/or medulla. Groups of mice were fed regular diet (CNT) or lithium-added diet (LI) or prasugrel in drinking water (PRSG) or a combination of Li in the diet and PRSG in drinking water (LI + PRSG) for 14 days and euthanized. Whole-tissue homogenates of renal medulla or cortex were processed for semiquantitative immunoblotting for abundances of sodium transporters, NKCC2 or NCC, and β-actin. a, b show immunoblot profiles for NKCC2 protein bands in the medulla and cortex, respectively, where each lane represents sample from one mouse. Bar graphs on the right side of each panel show corresponding densitometric values (mean ± SE) for the protein bands. *significantly different from the CNT group by Tukey-Kramer multiple comparison test following by ANOVA (P < 0.05). c immunoblot profile for NCC protein bands in the cortex, where each lane represents sample from one mouse. Bar graph on the right side of shows corresponding densitometric values (mean ± SE) for the immunoblot. *significantly different from the CNT and PRSG groups by Tukey-Kramer multiple comparison test following ANOVA (P < 0.03)