Fig. 6.

Effects of hyperosmotic exposure on P2X7 protein receptor levels. Stratified human corneal epithelial cells (a) and stratified human conjunctival epithelial cells (b) were treated with 90 and 120 mM NaCl-added media for 24 h. Cell lysates were successively immunoblotted with anti-P2X7 and anti-GAPDH receptor to confirm identical loading. The histograms indicate the levels of full-length and truncated form of P2X7 receptor. Data (mean ± standard deviation; n = 3) are given in arbitrary units (a.u.) and normalized to the intensity of the band corresponding to the full-length form (75 kDa band) in control cells. * p < 0.05, ** p < 0.01 and *** p < 0.001 vs control (75 kDa band). ^$ p < 0.05, ^$$ p < 0.01 vs control (45–42 kDa band)