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. 2017 May 15;7:1894. doi: 10.1038/s41598-017-02026-2

Table 1.

Ingredient composition and nutrient content of the experimental diets.

Item Starter (d 0–14) Grower (d15–28) Finisher (d29–43)
NTM LTM HTM NTM LTM HTM NTM LTM HTM
Wheat 47.30 47.14 47.18 45.17 45.01 45.05 50.77 50.61 50.65
Soybean meal, 47.5% CP 33.40 33.40 33.40 29.36 29.36 29.36 23.90 23.90 23.90
Barley 10.00 10.00 10.00 15.00 15.00 15.00 15.00 15.00 15.00
Soybean oil 4.45 4.45 4.45 6.30 6.30 6.30 6.30 6.30 6.30
L-lysine HCl 0.25 0.25 0.25 0.15 0.15 0.15 0.16 0.16 0.16
Methionine hydroxy analogue1 0.42 0.38 0.34 0.35 0.31 0.27 0.32 0.28 0.24
L-threonine 0.11 0.11 0.11 0.07 0.07 0.07 0.07 0.07 0.07
Dicalcium phosphate 18.5% 1.80 1.80 1.80 1.55 1.55 1.55 1.45 1.45 1.45
Limestone 1.25 1.25 1.25 1.05 1.05 1.05 1.05 1.05 1.05
Salt 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50
Choline cloride-60% 0.27 0.27 0.27 0.25 0.25 0.25 0.23 0.23 0.23
Mineral premix 0.202 0.203 0.202 0.203 0.202 0.203
Antioxidant4 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02
Fungicide5 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05
Coccidiostat6 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05
Bacitracin methylene disalicylate7 0.03 0.03 0.03 0.03 0.03 0.03 0.03 0.03 0.03
Vitamin premix8 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10
Calculated analysis
 ME, kcal/kg 3034 3034 3031 3155 3155 3153 3201 3199 3199
 Crude protein, % 22.8 22.7 22.7 21 21 21 19 19 19
 SID9 Lysine, % 1.27 1.27 1.27 1.1 1.1 1.1 0.97 0.97 0.97
 SID Methionine,% 0.64 0.64 0.64 0.56 0.56 0.56 0.5 0.5 0.5
 SID Sulfure amino acids, % 0.94 0.94 0.94 0.84 0.84 0.84 0.76 0.76 0.76
 SID Threonine, % 0.83 0.83 0.83 0.73 0.73 0.73 0.65 0.65 0.65
 Ca, % 1.05 1.05 1.04 0.9 0.9 0.89 0.86 0.86 0.85
 Available phosphorus, % 0.5 0.5 0.5 0.45 0.45 0.45 0.42 0.42 0.42

1Methionine hydroxy analogue (MHA®) (Novus International, Inc., St. Charles, MO).

2Mineral premix for LTM treatment supplied per kilogram of diet: FeSO4·H2O, 40 mg; Calcium Iodate, 1.25 mg; Sodium Selenite, 0.3 mg; Mintrex®Zn, 32 mg; Mintrex®Cu, 8 mg; Mintrex®Mn, 32 mg.

3Mineral premix for HTM treatment supplied per kilogram of diet: FeSO4·H2O, 40 mg; Calcium iodate, 1.25 mg; Sodium selenite, 0.3 mg; Mintrex®Zn, 64 mg; Mintrex®Cu, 16 mg; Mintrex®Mn, 64 mg.

4Santoquin® (Novus International, Inc., St. Charles, MO).

5Mold guard® (Kemin, Des Moines, Iowa).

6Coban®90 (Elanco Animal Health, Greenfield, IN).

7BMD® (Zoetis, Inc., Florham Park, NJ).

8Vitamin premix supplied per kilogram of diet: retinol, 9.2 mg; cholecalciferol, 100 µg; dl-α-tocopherol, 90 mg; menadione, 6 mg; thiamine, 6.2 mg; riboflavin, 26.5 mg; pantothenic acid, 39.7 mg; niacin, 100 mg; pyridoxine, 11 mg; folic acid, 4 mg; biotin, 0.3 mg; cyanocobalamin, 0.1 mg.

9Standardized ileal digestibility.