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. 2017 May 15;7:1906. doi: 10.1038/s41598-017-01936-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effects of NE on cell fibrosis and BNIP3L expression in CFs. Neonatal rat CFs were treated with different concentrations of NE for the indicated times. (a) Western blot analysis showing NE-induced fibronectin and collagen I expression. (b) Proliferation was measured using the MTT assay. (c) Cells were stained with PI and examined by FACS. The data were analyzed using the ModFit program. (d) Western blot analysis showing NE- induced BNIP3L expression. Each experiment repeated three times. Data shown are mean ± SD, *P < 0.05 vs. Control.