Table 6.
Primer sequences used to perform mutagenesis for each minigene and subcellular localization in the BMPR2 gene.
| Nucleotide change (Amino acid change) | Primers |
|---|---|
| c.156_157delTC (p.S52Sfs*2) | F 5′ → 3′: GGATAGGTGAGAGTAGAATCTCATGAAAATGGGACAATATTATG R 5′ → 3′: CATAATATTGTCCCATTTTCATGAGATTCTACTCTCACCTATCC |
| c.190A > C (p.S64R) | F 5′ → 3′: GGGACAATATTATGCTCGAAAGGTCGCACCTGCTATGG R 5′ → 3′: CCATAGCAGGTGCAACCTTTCGAGCATAATATTGTCCC |
| c.229A > T (p.I77L) | F 5′ → 3′: GGGAGAAATCAAAAGGGGACTTAAATCTTGTAAAACAAGGC R 5′ → 3′: GCCTTGTTTTACAAGATTTAAGTCCCCTTTTGATTTCTCCC |
| c.251G > T (p.C84F) | F 5′ → 3′: CTTTTTTGTATTCATATTGATTTATAGGATTTTGGTCTCACATTGGAG R 5′ → 3′: CTCCAATGTGAGACCAAAATCCTATAAATCAATATGAATACAAAAAAG |
| c.259G > T (p.H87Y) | F 5′ → 3′: ATCTTGTAAAACAAGGATGTTGGTCTTACATTGGAGATCCC R 5′ → 3′: GGGATCTCCAATGTAAGACCAACATCCTTGTTTTACAAGAT |
| c.275A > T (p.Q92L) | F 5′ → 3′: TCACATTGGAGATCCCCTAGAGTGTCACTATGAAG R 5′ → 3′: CTTCATAGTGACACTCTAGGGGATCTCCAATGTGA |
| c.327G > A (p.Q109Q) | F 5′ → 3′: CACTCCTCCCTCAATTCAAAATGGAACATACCGTTTC R 5′ → 3′: GAAACGGTATGTTCCATTTTGAATTGAGGGAGGAGTG |
| c.327G > C (p.Q109H) | F 5′ → 3′: CACTCCTCCCTCAATTCACAATGGAACATACCGTTTC R 5′ → 3′: GAAACGGTATGTTCCATTGTGAATTGAGGGAGGAGTG |
| c.412C > G (p.P138A) | F 5′ → 3′: CACCTCCTGACACAACAGCACTCAGTAAGTAAAGT R 5′ → 3′: ACTTTACTTACTGAGTGCTGTTGTGTCAGGAGGTG |
| c.484G > C (p.A162P) | F 5′ → 3′: TGGCATCAGTCTCTGTATTACCTGTTTTGATAGTTGCC R 5′ → 3′: GGCAACTATCAAAACAGGTAATACAGAGACTGATGCCA |
| c.600A > C (p.L200L) | F 5′→ 3′: CCAACAGTTTCAGATTATCGAGATCAAGAGAGGGTTCGG R 5′ → 3′: CCGAACCCTCTCTTGATCTCGATAATCTGAAACTGTTGG |
| c.633A > G (p.R211R) | F 5′ → 3′: CAGCTGATTGGCCGGGGTCGATATGGAGC R 5′ → 3′: GCTCCATATCGACCCCGGCCAATCAGCTG |
| c.637C > A (p.R213R) | F 5′ → 3′: AGCTGATTGGCCGAGGTAGATATGGAGCAGTATAT R 5′ → 3′: ATATACTGCTCCATATCTACCTCGGCCAATCAGCT |
| c.654T > A (p.Y218*) | F 5′ → 3′: CGAGGTCGATATGGAGCAGTATAAAAAGGCTCCTTGG R 5′ → 3′: CCAAGGAGCCTTTTTATACTGCTCCATATCGACCTCG |
| c.742A > G (p.R248G) | F 5′ → 3′: AATTTTATCAACGAAAAGAACATTTACGGAGTGCCTTTGATGGAAC R 5′ → 3′: GTTCCATCAAAGGCACTCCGTAAATGTTCTTTTCGTTGATAAAATT |
| c.790G > A (p.D264N) | F 5′ → 3′: CCCGCTTTATAGTTGGAAATGAGAGAGTCACTGCA R 5′ → 3′: TGCAGTGACTCTCTCATTTCCAACTATAAAGCGGG |
| c.835G > T (p.V278V) | F 5′ → 3′: CGCATGGAATATTTGCTTGTTATGGAGTACTATCCCAATGT R 5′ → 3′: ACATTGGGATAGTACTCCATAACAAGCAAATATTCCATGCG |
| c.893G > A (p.W298*) | F 5′ → 3′: ATTTAAGTCTCCACACAAGTGACTAGGTAAGCTCTTGC R 5′ → 3′: GCAAGAGCTTACCTAGTCACTTGTGTGGAGACTTAAAT |
| c.981 T > C (p.P327P) | F 5′ → 3′: ATCCAAACAGATCATTATAAACCCGCAATTTCCCATCGAGATTTAAA R 5′ → 3′: TTTAAATCTCGATGGGAAATTGCGGGTTTATAATGATCTGTTTGGAT |
| c.1021G > A (p.V341M) | F 5′ → 3′: GAGATTTAAACAGCAGAAATGTCCTAATGAAAAATGATGGAACCTG R 5′ → 3′: CAGGTTCCATCATTTTTCATTAGGACATTTCTGCTGTTTAAATCTC |
| c.1400A > G (p.K467R) | F 5′ → 3′: CTTACCAGGCTATTTTCCTTCCAGGCTTCTGGGAA R 5′ → 3′: TTCCCAGAAGCCTGGAAGGAAAATAGCCTGGTAAG |
| c.1467G > A (p.E489E) | F 5′ → 3′: GTTGGGACCAGGATGCAGAAGCTCGGCTTA R 5′ → 3′: TAAGCCGAGCTTCTGCATCCTGGTCCCAAC |
F: Forward; R: Reverse.