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. 2017 May 15;7:1908. doi: 10.1038/s41598-017-02017-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of virus accumulation in protoplasts from wild type (WT) and HC-Pro Nicotiana benthamiana. Quantitative Reverse transcriptase PCR (qRT-PCR) data from WT and HC-Pro N. benthamiana protoplasts were used to calculate fold change of viral RNAs at T0 and T48 time points. In panels A data for the Totivirus Penicillium aurantiogriseum totivirus 1 (PaTV1) are reported: for WT protoplasts we observed ca. 5 times more RNA at T48 (yellow bar) time point and ca. 15 times more RNA in HC-Pro protoplasts (orange bar). In panels C quantification of the Partitivirus Penicillium aurantiogriseum partitivirus 1 (PaPV1): no statistically significant differences were observed for WT protoplasts, but ca. 10 times more viral RNA was observed in case of HC-Pro protoplasts. Panels B, D, E and F show data for the other viruses: none of them displays a statistically significant increase in virus RNA load. *P < 0.05 **P < 0.01 and ***P < 0.001.