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. 2017 May 15;7:1885. doi: 10.1038/s41598-017-01764-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Integrin beta8 regulates the activity of FAK in the endometrial epithelial cells during their receptivity. (A) Integrin beta8 expression analysis was performed on the endometrial epithelial cells with the help of immunoblotting and densitometry. (B) Employing immunofluroescence, the localization of integrin beta8 was performed through confocal laser scanning microscope in the endometrial epithelial cells of mouse origin. (C) Silencing of Itgb8 from endometrial epithelial cells was confirmed by immunoblotting and densitometry. (D) The expression level of phosphorylated and total forms of FAK was examined in response to Itgb8 silencing in the endometrial epithelial cells using immunoblotting and densitometry. (E,F) Similarly, FAK activity was determined by way of phosphorylation (Y-397) level determination by ELISA in the endometrial tissue protein extract from day5, 1000 h stage post-Itgb8 silencing at day4, 1000 h. Actin beta was used as the loading control to normalize the western blot values. (**p < 0.01,*p < 0.05, NS p > 0.05). Magnification of the image was at 63X and 189X. Scale bar; 20 and 5 µm respectively.