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. 2017 May 15;7:1885. doi: 10.1038/s41598-017-01764-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Integrin beta8 and FAK participate in the blastocyst attachment reaction. (A) Attachment of human placenta origin JAr cells spheroids on the endometrial epithelial cells (Ishikawa) was analyzed post-Itgb8 silencing from endometrial cells in percentage. The percentage spheroids adhered/attached were determined after the 24 hrs incubation. (B) This was accompanied by the analysis of Itgb8 knockdown efficiency in the Ishikawa cells by immunoblotting and densitometry. (C) Post-FAK activity (tyrosine 397 phosphorylation) inhibition in the Ishikawa cells, JAr cells spheroids attachment were seen and analyzed in the form of the percentage. (D) The activity of FAK (phosphorylation) was determined in the Ishikawa cells post-FAK activity inhibition employing ELISA based method. (***p < 0.001, **p < 0.01,*p < 0.05). Scale bars: 200 μm (40X).