Figure 3.

BMDM response to stimulation is moesin-independent. (A) TNF secretion upon BMDM stimulation determined in supernatants after 24 h stimulation by TNF-ELISA and CBA, n = 4 for untreated/LPS/P. falciparum schizont extract (PfSE), n = 1 for P. berghei schizont extract (PbSE), and P. berghei schizonts (PbS); unpaired t-test revealed that differences are not significant (ns); (B,C) Tnf and Il1b expression detected by qPCR, relative expression was calculated with respect to Gapdh, relative expression in treated groups was normalized to untreated cells; n = 2 for LPS/PbS/PbSE, n = 1 for PfSE; LPS: 10 ng/ml, PfSE: 1:100 dilution in medium, PbSE: 1:1,000 dilution in medium, PbS added at a ratio of 1:10. Bars indicate mean + SD.