Figure 7.

Moesin-deficiency does not protect mice from P. berghei ANKA-induced ECM. Moesin-deficient mice and corresponding WT controls were infected by i.v. injection of 10,000 P. berghei ANKA Bergreen-infected erythrocytes and the impact of moesin-deficiency on the course of infection and the systemic immune response was analyzed; (A) Survival curve indicating development of ECM in both WT and moesin-deficient mice, n = 12/genotype; Mantel Cox test revealed that differences are non-significant. (B) Representative parasitemia (n = 6/genotype) indicating similar progression of parasite growth independent of the host genotype. Parasitemia was determined by flow cytometric analysis of GFP⁺ erythrocytes; Dots display mean parasitemia + SD (C–F) Serum was collected from infected mice (n = 6 for WT, n = 5 for Msn^−/−) at days 3, 5, and 7 post-infection by retrobulbar punction and analyzed for serum concentration of TNF (C), IFN-γ (D), IL-6 (E), and CCL2 (F) by CBA. Dots represent individual mice, bars indicate means. Analysis by Mann-Whitney test: ^*P < 0.05, ns, non-significant.