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. 2017 May 16;8:856. doi: 10.3389/fmicb.2017.00856

FIGURE 2.

FIGURE 2

Diagram of the amount of biofilm-forming microorganisms (S. mutans) (log CFU/ml) with respect to microbial biomass (S. mutans) using the method of crystal violet (CV) staining at particular time points of single-species biofilm formation. The analyzed features had no normal distribution (p < 0.05, Shapiro–Wilk test), so a Spearman correlation coefficient was used for the analysis. The correlation between the amount of microorganisms expressed as log CFU/ml and the biomass of the formed biofilm expressed as OD (optical density measured at a wavelength λmax = 540 nm) is statistically significant at each time point (p < 0.05). These dependencies are positive, i.e., the higher the log CFU/ml, the higher the OD and vice versa.