Table 3.
CphA gene variants identified in Aeromonas spp. isolated in this study.
| Aeromonas spp. strain | cphA variant∗ | BLAST N∗∗ | BLAST X∗∗∗ | |
|---|---|---|---|---|
| Influent | 6.28 | cphA1/cphA | 95%, A. salmonicida (NG_047668.1) | 99%, A. salmonicida (WP_059112452.1) |
| 7.47 | cphA/cphA | 96%, A. aquariorum (JF972621.1) | 98%, A. hydrophila (WP_024941978.1) | |
| WL1 | cphA1/cphA | 95%, A. salmonicida (NG_047668.1) | 99%, A. salmonicida (WP_058395305.1) | |
| WL3 | cphA1/cphA | 96%, A. salmonicida (NG_047668.1) | 99%, A. salmonicida (WP_058395305.1) | |
| Activated sludge | T6 | cphA/subclass B2 metallo-β-lactamase | 96%, A. aquariorum (JF972618.1) | 99%, A. hydrophila (WP_050559324.1) |
| S6 | cphA1/cphA | 94%, A. salmonicida (NG_047668.1) | 96%, A. australiensis (WP_040097284.1) | |
| S12 | cphA/cphA2 | 96%, A. aquariorum (JF972621.1) | 98%, A. hydrophila (WP_063865208.1) | |
| E34 | cphA/cphA | 96%, A. veronii (JF972616.1) | 97%, A. australiensis (WP_040097284.1) | |
| 203 | cphA7/cphA7 | 97%, A. jandaei (NG_050400.1) | 93%, A. jandaei (WP_063865212.1) | |
| 206 | cphA7/cphA | 96%, A. jandaei (NG_050400.1) | 96%, A. hydrophila (WP_049047121.1) | |
| 221 | cphA7/cphA | 96%, A. jandaei (NG_050400.1) | 99%, A. hydrophila (WP_060390377.1) | |
| 343A | cphA7/cphA | 96%, A. jandaei (NG_050400.1) | 99%, A. hydrophila (WP_060390377.1) | |
| 357A | cphA7/cphA | 96%, A. jandaei (NG_050400.1) | 99%, A. hydrophila (WP_060390377.1) | |
| 364B | cphA7/cphA | 95%, A. jandaei (NG_050400.1) | 96%, A. hydrophila (WP_049047121.1) | |
| 368A | cphA7/cphA | 96%, A. jandaei (NG_050400.1) | 95%, A. hydrophila (WP_049047121.1) | |
| 280 | cphA/cphA1 | 96% A. aquariorum (JF972625.1) | 97%, A. hydrophila (WP_063844282.1) | |
| 297 | cphA/cphA1 | 96% A. aquariorum (JF972625.1) | 100%, A. hydrophila (WP_060390377.1) | |
| KO26 | cphA/cphA | 94%, A. aquariorum (JF972618.1) | 97%, A. salmonicida (WP_043135519.1) | |
| AKO1 | cphA/cphA | 96%, A. aquariorum (JF972618.1) | 97%, A. veronii (WP_005343384.1) | |
| AKO16 | cphA-like/ cphA-like | 100%, Aeromonas sp. G.I10.28 (DQ447638.1) | 100%, Aeromonas sp. G.I10.28 (ABE01851.1) | |
| AKP15 | cphA/cphA1 | 99%, A. hydrophila (NG_047667.1) | 99%, A. hydrophila (WP_063844282.1) | |
| AKP19 | cphA-type/cphA | 96%, A. dhakensis (AB765398.1) | 99%, A. hydrophila (WP_043161524.1) | |
| AKP23 | cphA2/cphA | 97%, A. hydrophila (NG_050396.1) | 99%, A. hydrophila (WP_043161524.1) | |
| Effluent | 426 | cphA1/cphA | 97%, A. hydrophila (NG_047671.1) | 100%, A. hydrophila (WP_024945765.1) |
| 481 | cphA1/cphA | 96%, A. hydrophila (NG_047671.1) | 100%, A. hydrophila (WP_024945765.1) | |
| 483 | cphA1/cphA | 97%, A. hydrophila (NG_047671.1) | 100%, A. hydrophila (WP_024945765.1) | |
| WY39 | cphA/cphA | 94%, A. aquariorum (JF972621.1) | 96%, A. allosaccharophila (WP_042063527.1) | |
| WY47 | cphA1/cphA | 96%, A. veronii (NG_047669.1) | 97%, A. australiensis (WP_040097284.1) | |
| AWY14 | cphA/cphA | 97%, A. veronii (JQ814285.1) | 98%, A. australiensis (WP_040097284.1) |
∗Gene variant based on BLAST N/BLAST X; ∗∗Identity, bacterial species and accession number of the most identical alignments using BLAST N; ∗∗∗Identity, bacterial species and accession number of the most identical alignments using BLAST X.