FIGURE 5.

Detection of GFP-tagged BaMV in the regurgitant and excretion fluids of dipteran insects. To investigate whether BaMV RNA could pass through the digestive systems of diptera insects, the reguritant and excretion fluids (as indicated on the top of the lanes) were collected from A. orientalis (A) and G. fasciventris (B) fed with GFP-tagged BaMV-containing media and subjected to RT-PCR analysis for the presence of BaMV RNA, using different primer pairs (as indicated at the bottom of each gel). To test whether the walls of the feeding tube were contaminated during the feeding process, aliquots of nucleic acid extraction buffer (2 μl) were used to elute materials surrounding the regurgitant or excretion droplets, and subjected to RT-PCR analysis concurrently (lanes labeled “Wall”). For the positive control, 1 μl of GFP-BaMV-supplemented medium was assayed concurrently. Lane M, size marker.