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. 2017 May 16;10:125. doi: 10.3389/fnmol.2017.00125

Figure 5.

Figure 5

Action of NFA on G254A-α1 GlyR. (A) Amino acid sequences of the TM2 domain of α1 wild type and mutant subunits (mutated residue is highlighted by red) and recordings of inhibition of glycine-induced currents (30 μM) by different concentrations of NFA (30, 100, and 300 μM); Vhold is +30 mV (upper traces) and −30 mV (bottom traces). (B) Representative current-voltage relationships recorded during application of glycine (30 μM) alone or in the presence of different concentrations of NFA. (C) NFA IC50 at different potentials, currents were induced by application of 30 μM (gray, n = 13) and 100 μM (green, n = 8) of glycine. (D) Comparison of NFA sensitivities of α1 GlyR wildtype (white, n = 8) and G254A-α1 GlyR (black, n = 13). Currents were induced by 30 μM glycine, ***p < 0.001, unpaired t-test. Data represented as mean ± SEM.