Table 1.
Bacterial strains and plasmids used in this study.
| Strain or plasmid | Relevant characteristic | Source or reference |
|---|---|---|
| Bacterial strains | ||
| Escherichia coli | ||
| WM6026 | Donor strain for conjugation; lacIq, rrnB3, DElacZ4787, hsdR514, DE(araBAD)567, E(rhaBAD)568, rph-1, att-lambda::pAE12-del(oriR6K-cat::frt5), DE(endA)::frt, uidA(delMluI)::pir(wt), attHK::pJK1006-del1/2 (deloriR6K-cat::frt5, deltrfA::frt) | William Metcalf, University of Illinois |
| BL21 (DE3) | F- ompT hsdR17(rB- mB+) gal dcm(DE3) F-, ompT, hsdSB(rB- mB-), gal(aaacI 857, ind1, Sam7, nin5, lacUV5-T7gene1), dcm(DE3) | Novagen |
| Shewanella oneidensis | ||
| MR-1 | Wild-type | ATCC (3) |
| Δcrp | The crp gene (SO_0624) disrupted | 11 |
| Plasmids | ||
| pMElacZ | pME4510 derivative, lacZ Gmr | 24 |
| pMElldP+1 | pMElacZ containing the region from +1 to +192 relative to TSPlldP | This study |
| pMElldP-60 | pMElacZ containing the region from –60 to +192 relative to TSPlldP | This study |
| pMElldP-182 | pMElacZ containing the region from –182 to +192 relative to TSPlldP | This study |
| pMElldP-541 | pMElacZ containing the region from –541 to +192 relative to TSPlldP | This study |
| pBBR1MCS-2 | Broad-host-range vector, lacZ promoter, Kmr | 22 |
| pBBR1MCS-5 | Broad-host-range vector, lacZ promoter, Gmr | 22 |
| pBBRcrp | pBBR1MCS-5 containing crp | This study |
| pBBRdld | pBBR1MCS-2 containing dld | This study |
| pET-crp | pET-28(a)-based plasmid expressing N-his-crp | 11 |