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. 2017 May 16;8:306. doi: 10.3389/fphys.2017.00306

Table 5.

Numbers of genes and proteins associated with cytoskeletal-muscle fibers, phosphorylation regulation, cellular quality control mechanisms (proteosome and autophagy) that were higher (H) or lower (L) in breast muscle of high feed efficiency (FE) compared to low FE pedigree broiler male for the terms provided obtained from global expression microarray, proteomics, and RNAseq datasets and for numbers of all three global expression datasets combined.

Microarraya Proteomicsb RNAseqc
Term H L P* H L P* H L P* H
PHOSPHORYLATION
Cytoskeletal-muscle fibersd 9 20 0.0004 7 21 0.0044 21 42 0.0030 35
Protein tyrosine phosphatase 2 1 2 0 5 27 0.0020 9
Protein phosphatase 4 2 7 1 0.0313 30 24 0.0778 41
Tyrosine kinase 6 4 1 0 11 31 0.001 17
QUALITY CONTROL
Proteosome 7 3 0.1172 21 4 0.0004 31 4 2E-06 59
Autophagy 6 2 0.1093 4 0 0.0625 36 15 0.0014 49
a

cDNA microarray—Kong et al. (2011).

b

Shotgun proteomics—Kong B. et al. (2016).

c

RNAseq—Bottje et al. (2017).

d

Actin (ACTA), myosin (MYH), troponin (TNNT).

*

Binomial (exact) P values for H vs. L in each dataset. Blanks are indicative of P > 0.10.

P values not underlined indicate a significant bionomial skew in high FE.

P values that are underlined indicate a significant skew in low FE.

P values that are blank are indicative of values > 0.10.