Table-I.

Primers set and conditions used for PCR in this study.

Genes	Nucleotide sequence (5'-3')	PCR product (bp)	PCR conditions	Reference
16S rRNA	GCGACCTGCTGGAACATTAC CGTTAGCTGCATTACTGGAGA	138 bP	95ºC, 50 s; 60ºC, 50s; 72ºC, 50 s (35 cycles)	28
glmM	AAGCTTTTAGGGGTGTTAGGGGTTT AAGCTTACTTTCTAACACTAACGC	294 bp	95ºC, 50 s; 56ºC, 50s; 72ºC, 50 s (35 cycles)	23
babA2	CCAAACGAAACAAAAAGCGT GCTTGTGTAAAAGCCGTCGT	271 bp	95ºC, 50 s; 44ºC, 50s; 72ºC,50 s (35 cycles)	29
iceA1	GTGTTTTTAACCAAAGTATC CTATAGCCATTATCTTTGCA	247 bp	95ºC, 50 s; 57ºC, 50s; 72ºC,50 s (35 cycles)	29
iceA2	GTTGGGTATATCACAATTTAT TTTCCCTATTTTCTAGTAGGT	229 bp	95ºC, 50 s; 57ºC, 50s; 72ºC,50 s (35 cycles)	28
cagA	TTGACCAACAACCACAAACCGAAG CTTCCCTTAATTGCGAGATTCC	183 bp	95ºC, 50 s; 60ºC, 50s; 72ºC,50 s (35 cycles)	35
vacA s1/s2	ATGGAAATACAACAAACACAC CTGCTTGAATGCGCCAAAC	259/286 bp	95ºC, 50 s; 58ºC, 50s; 72ºC,50 s (35 cycles)	20
vacA m1/m2	CAATCTGTCCAATCAAGCGAG GCGTCAAAATAATTCCAAGG	567/642 bp	95ºC, 50 s; 54ºC, 50s; 72ºC,50 s (35 cycles)	14