Figure 1.

Rapid purification and in vitro processing of adult hSk-SCs does not alter basic SC characteristics. A, Timeline of isolation, purification, and expansion of skin-derived SCs. B–E, Representative bright field images of skin-derived SCs during the initial stages of isolation, purification, and expansion. F, Chromosomes isolated from a representative adult hSk-SC that had been previously passaged and expanded in vitro for five weeks. G–I, Representative immunocytochemical images and quantification of adult human nerve (hN-SC)- and skin (hSk-SC)-derived SCs revealed that the majority of cells in culture expressed SOX10, NES, s100, and p75. Scale bar, 25 μm. All representative images are of cells derived from a 46-year-old male.