Figure 5.

Nerve and skin-derived SCs produce growth factors that promote neurite outgrowth ex vivo. Representative immunohistochemical images of rat DRG explants immunostained for βIII tubulin (red) and Hoechst (blue). Explants were treated with (A) SC base media, (B) nerve-derived SC-conditioned media, or (C) skin-derived SC-conditioned media. D, Quantification of the total number of neurites from 200 to 2000 μm from the explant. n = 7–8 individual explants per patient (n = 3–4 patient media per group). Data are shown as mean ± SEM. Data were analyzed with one-way ANOVA with Tukey post hoc test comparison to base SC media. ***p < 0.001. Scale bar, 500 µm.