Table 1.

Sequences of adaptors and primers for methylation-sensitive amplification polymorphism analysis

Adaptors and primers	EcoRI(E)	HpaII/MspI(HM)
Adaptor 1	5'-CTCGTAGACTGCGTACC-3'	5'-GACGATGAGTCCTGAG-3'
Adaptor 2	5'-AATTGGTACGCAGTC-3'	5'-CGCTCAGGACTCAT-3'
Amplification primer	5'-GACTGCGTACCAATTC-3'	5'-GATGAGTCCTGAGCGG-3'
Selective	5'-GACTGCGTACCAATTCCA-3'(E3)	5'-GATGAGTCCTGAGCGGCAC-3'(HM2)
Amplification	5'-GACTGCGTACCAATTCAG-3'(E4)	5'-GATGAGTCCTGAGCGGCTA-3'(HM5)
Primer	5'-GACTGCGTACCAATTCAA-3'(E5)	5'-GATGAGTCCTGAGCGGCTC-3'(HM6)
		5'-GATGAGTCCTGAGCGGCTG-3'(HM7)