Table 2.
Source of variation | Total rate of methylation (%) | Mean | Variance test | |||||
---|---|---|---|---|---|---|---|---|
Age | E3HM2 | E3HM6 | E3HM7 | E4HM5 | E4HM6 | E5HM5 | ||
2 | 14.28 | 15.38 | 10.63 | 17.46 | 19.4 | 32.5 | 18.28 ± 7.58a* | SS=0.263, df=8, MS=0.033, F=14.231, Sig=0.000, P<0.01 |
6 | 14.28 | 13.72 | 15.38 | 24.24 | 23.19 | 32.91 | 20.62 ± 7.56a | |
7 | 12 | 14.28 | 21.56 | 20.31 | 24.24 | 30.49 | 20.48 ± 6.72a | |
13 | 14.28 | 20.41 | 10.71 | 20.28 | 22.39 | 37.35 | 20.90 ± 9.18ab | |
18 | 18.18 | 26.41 | 23.64 | 23.08 | 30.77 | 39.24 | 26.89 ± 7.33bc | |
32 | 25 | 31.48 | 25.45 | 27.69 | 28.36 | 44.3 | 30.38 ± 7.20bc | |
34 | 23.07 | 25.92 | 28.84 | 26.56 | 29.23 | 36.25 | 28.31 ± 4.48bc | |
44 | 20.83 | 25 | 31.48 | 32.81 | 31.81 | 36.25 | 29.70 ± 5.67bc | |
63 | 26.66 | 50 | 37.04 | 32.81 | 30.76 | 50 | 37.88 ± 9.97cd |
Total rate of methylation (%) = Total number of methylation bands/Total number of amplified bands × 100%; SS represents Stdev square, df represents degree freedom, MS represents Mean square.
*Values followed by different small letters indicate L.S.D significant difference at p<0.05.