FIG 5.

Neutralization of MeV by anti-MeV-H antibodies from pooled human sera. (A) Reactivity of pooled human sera after 0 days (untreated) or 4 days (MeV-F absorbed) of incubation at 37°C with cells expressing MeV-F. Binding reactivity was measured by fluorescence-activated cell sorter analysis using MeL-JuSo cells expressing or not expressing the MeV glycoproteins (MeV-H and MeV-F). Anti-human immunoglobulin G(H+L) Alexa Fluor-488 was used for staining. (B) Neutralization of MeV by anti-H pooled human sera. The IC₅₀s and standard deviations (StDev) were calculated from 3 experiments performed independently in quadruplicate. Values were calculated after nonlinear regression of the corresponding neutralization curves with GraphPad Prism software. CDV, canine distemper virus; NA, not applicable.