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. 2017 May 12;91(11):e00250-17. doi: 10.1128/JVI.00250-17

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Copyright © 2017 Yoshikawa et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 4 — Conservation of reduced degradation activity of FIV subtype B Vif against feline A3. The expression plasmids for Flag-tagged FIV Vif were cotransfected with or without the expression plasmids for feline A3Z2Z3 tagged with HA. Representative results of Western blotting assays (A) and FIV reporter assays (B) are shown. FIV infectivity is shown as the percentage of the value for no A3Z2Z3. * in panel B, P < 0.05 versus Petaluma. The assays were independently performed in triplicate. Data represent averages with SDs. (C) Co-IP assay. The expression plasmids for His-tagged FIV Vif were cotransfected with or without the expression plasmids for feline A3Z2Z3 tagged with HA, and the co-IP assay was performed as described in Materials and Methods. Representative results from co-IP assays using an anti-HA antibody (top) and cell lysate (bottom) are shown.