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Affinity of the NTD and its mutants for nucleic acida
| NTD variants | FP |
MST |
||
|---|---|---|---|---|
| Kd (μM) | SD | Kd (μM) | SD | |
| WT | 2.3 | 0.2 | 6.0 | 0.9 |
| R63A | 6.5 | 0.4 | 11.4 | 1.9 |
| K75A | 3.3 | 2.7 | 13.8 | 2.2 |
| K121A | 5.6 | 0.8 | 17.6 | 2.7 |
| R61A | 7.0 | 0.6 | 24.0 | 4.0 |
| R116A | 6.7 | 0.8 | 27.5 | 4.7 |
| H77A | 5.7 | 1.0 | 40.2 | 5.4 |
| Q59A | 7.3 | 0.1 | 43.1 | 5.5 |
| Q59A/H77A | 9.7 | 1.1 | 47.3 | 8.9 |
| R61A/K75A | 11.8 | 2.8 | 55.4 | 8.0 |
| H77A/R116A | 11.8 | 1.9 | 72.4 | 11.7 |
The affinities were determined by fluorescence polarization (FP) and microscale thermophoresis (MST). WT, wild type.
