ADCC activity of cAbs and human Abs. (A) Gating strategy and normalization of the numbers of viable target cells using a representative cAb 65C6/IgG2a as an example. (B) Calculation of percentage of ADCC using a representative cAb 65C6/IgG2a as an example. T, target cells; E, effector cells. (C to E) Percentage of ADCC by 100F4/IgG2a and 100F4/D265A along with VRC01 control against CEM.NKR cells transduced with GPI-HA from virus strains SZ06 (C), NE14 (D), and SX06 (E). (F to H) Percentage of ADCC by 65C6/IgG2a and 65C6/D265A along with VRC01 control against CEM.NKR cells transduced with GPI-HA from SZ06 (F), NE14 (G), and SX06 (H). (I to K) Percentage of ADCC by human Abs 100F4 and 65C6 along with VRC01 control against CEM.NKR cells transduced with GPI-HA from SZ06 (I), NE14 (J), and SX06 (K). *, P < 0.05; **, P < 0.01; ns, not significant.