TABLE 2.
Summary of the results of ELISA binding, neutralization, FACS analysis, ADCC, and in vivo testing of chimeric Abs 100F4/IgG2a, 100F4/D265A, 65C6/IgG2a, and 65C6/D265A
| Parameter and cAb | Value for parameter in virus straina: |
||
|---|---|---|---|
| SZ06 | NE14 | SX06 | |
| IC50 (μg/ml) | |||
| 100F4/IgG2a | 0.009 | 0.010 | ND |
| 100F4/D265A | 0.007 | 0.010 | ND |
| 65C6/IgG2a | 0.008 | 0.014 | 20 |
| 65C6/D265A | 0.004 | 0.005 | 21 |
| EC50 (ng/ml) | |||
| 100F4/IgG2a | 4.4 | 7.3 | 417.0 |
| 100F4/D265A | 13.1 | 27.0 | 340.0 |
| 65C6/IgG2a | 13.6 | 23.8 | 446.0 |
| 65C6/D265A | 8.1 | 8.8 | 381.0 |
| MFIb | |||
| 100F4/IgG2a | 254 | 155 | 137 |
| 100F4/D265A | 216 | 145 | 127 |
| 65C6/IgG2a | 290 | 214 | 128 |
| 65C6/D265A | 294 | 260 | 138 |
| ADCC (%) (mean ± SEM) | |||
| 100F4/IgG2a | 18.2 ± 2.6 | 21.2 ± 2.5 | 24.1 ± 6.6 |
| 100F4/D265A | 5.0 ± 3.1 | 0.3 ± 0.4 | 2.4 ± 3.2 |
| 65C6/IgG2a | 20.1 ± 2.7 | 13.9 ± 2.0 | 17.2 ± 2.8 |
| 65C6/D265A | 1.5 ± 3.4 | 5.5 ± 1.7 | −0.6 ± 5.5 |
| Survival rate (no. of mice that survived/total no. of mice) | |||
| 100F4/IgG2a | 4/5 | 5/5 | 5/5 |
| 100F4/D265A | 0/5 | 1/5 | 1/5 |
| 65C6/IgG2a | 4/5 | 5/5 | 5/5 |
| 65C6/D265A | 4/5 | 5/5 | 5/5 |
a
ND, not detected.
b
MFI, median fluorescence intensity of FACS staining by chimeric Abs.