FIG 7.

Electron micrographs of CAD neurons infected with the HSV F-gE/US9-GFP double mutant. CAD neurons growing on collagen-coated 60-mm dishes were differentiated for 7 days, infected with the F-gE/US9-GFP double mutant at 20 PFU/cell for 18 h, and then fixed and processed for electron microscopy. CAD neurons infected with F-gE/US9-GFP exhibited few enveloped virions on the cell surface (A) and numerous unenveloped (asterisks in panels B and C) and partially enveloped (black arrows in panels B and C) capsids in the cytoplasm. The cell surface is indicated in panel A.