FIG 9.

Production of infectious virus following infection of CAD neurons with an HSV double mutant (F-gE/US9-GFP) or repaired virus (F-gE/US9-GFP-R). CAD neurons were grown on polylysine/laminin-coated 6-well (10 cm²) dishes and then differentiated for 7 days. The cells were infected (in triplicate) with F-gE/US9-GFP or the repaired virus (F-gE/US9-GFP-R) at 20 PFU/cell for 2 h and then washed extensively and incubated for 2, 8, 12, 18, or 22 h. The combined cells and culture media were collected and sonicated, and the amount of infectious virus was determined by plaque titration on Vero cells.