TABLE 4.
Cytoplasmic and cell surface distribution of capsids produced by gE− US9−, gE-null, and US9-null mutants in HaCaT human keratinocytesa
| Virus | No. of capsids (% of all capsids) |
|||
|---|---|---|---|---|
| Unenveloped cytoplasmic | Partially enveloped cytoplasmic | Enveloped cytoplasmic | Cell surface virion | |
| F-gE/US9-GFP-R | 41 (5.9) | 15 (2.1) | 40 (5.7) | 593 (85) |
| F-gE/US9-GFP | 74 (15) | 39 (7.7) | 78 (15) | 314 (62) |
| F-US9-GFP | 63 (8.9) | 7 (1.0) | 43 (6.1) | 591 (84) |
| F-gEβ | 89 (16) | 23 (4.1) | 51 (9.2) | 392 (71) |
a
EM images of sections of 8 to 11 HSV-infected cells were analyzed by counting and characterizing the capsids.