FIG 2.

CXCR5 transduction of primary rhesus macaque T cells confers functional CXCL13-mediated signaling. Analyses of CXCR5-transduced CD8 T cells are presented. (A) Dot plot of CD8/CXCR5 flow cytometry. (B) Near-infrared LI-COR ERK1/2 and phosphorylated ERK1/2 (pERK1/2) immunoblots of cell lysates. The CXCL13 exposure time (in minutes) is indicated above each sample. The positions of molecular mass standards (in kilodaltons) are indicated to the left of the blot, and the positions of bands are identified to the right of the blot. α-ERK1/2, ant-ERK1/2 antibody. (C) Graph of the kinetics of pERK1/2 induction. (D) Graph of cell counts from CXCL13-induced migration of transduced cells in a transwell assay.