TABLE 1.
Summary of data collection, image-processing parameters, and refinement statistics
| Parameter | HBoV1 | HBoV3 | HBoV4 |
|---|---|---|---|
| Total no. of micrographs | 3,519 | 1,719 | 1,494 |
| Defocus range (μm) | 0.79–4.43 | 0.82–3.91 | 0.90–4.26 |
| Electron dose (e−/Å2) | 63 | 63 | 60 |
| No. of frames/micrograph | 19 | 34 | 38 |
| Pixel size (Å/pixel) | 0.95 | 0.95 | 0.95 |
| Starting no. of particles | 51,756 | 31,896 | 14,882 |
| No. of particles used for final map | 31,064 | 28,707 | 13,394 |
| Inverse B factor used for final map (Å2) | 1/100 | 1/100 | 1/100 |
| Resolution of final map (Å) | 2.9 | 2.8 | 3.0 |
| PHENIX model refinement statistics | |||
| Residue range | 33–542 | 34–539 | 34–541 |
| Map CC | 0.819 | 0.837 | 0.810 |
| RMSD (Å) | |||
| Bonds | 0.01 | 0.01 | 0.01 |
| Angles | 1.46 | 1.15 | 1.33 |
| All-atom clash score | 20.36 | 16.57 | 23.39 |
| Ramachandran plot (%) | |||
| Favored | 85.7 | 82.1 | 80.6 |
| Allowed | 12.9 | 15.5 | 16.6 |
| Outliers | 1.4 | 2.4 | 2.8 |
| Rotamer outliers | 0.0 | 0.0 | 0.0 |
| No. of Cβ deviations | 0 | 0 | 0 |