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Effect of temperature on SLA80-NS5 binding. Shown are fluorescence titrations of fluorescein-labeled SLA80 with NS5 (λex = 480 nm; λem = 520 nm) in buffer (50 mM Tris [pH 7.5], 100 mM NaCl, 1 mM MgCl2) at 10, 20, and 25°C. The concentration of fluorescein-labeled SLA80 is 1.5 × 10−8 M. The solid line is a nonlinear least-squares fit of both titration curves with a K of 5.5 × 106 M−1.
