FIG 2.
Western blot comparison of PrPTSE patterns in the brains of the three TgHu mouse genotypes and in the corresponding CJD inocula. Mice inoculated with sCJD-MV1 (lanes 2 to 4) produced a PrPTSE type identical to that of the original inoculum (lane 1) regardless of the genotype. In the MV2 group (lanes 5 to 8), only the HuVV genotype (lane 8) reproduced the original type 2 PrPTSE (lane 5). Atypical CJD PrPTSE MVAG (lane 9) with features identical to those of the original inoculum was observed only in HuVV mice (lane 12). No PrPTSE was detected in HuMM and HuMV mice injected with CJD-MVAG (lanes 10 and 11). Approximate molecular masses (kilodaltons) are on the right. The membranes were probed with 3F4.