FIG 1.

Differential sensitivity of BST-2 isoforms to HIV-1 Vpu in Jurkat cell lines. Jurkat Tag cells (A and D) expressing no BST-2 (Jurkat Tag EV [empty vector]) or stably expressing the L-BST2 (B and D) or S-BST-2 (C and D) were mock infected or infected with the transmitted/founder virus HIV-1 CH58 (CH58TF) expressing Vpu (wild-type CH58TF [CH58TF wt]) or not expressing Vpu (CH58TF Vpu−). Forty-eight hours postinfection, cells were stained with anti-BST-2 Ab, followed with appropriate secondary Abs. (A to C) Histograms depicting representative staining; (D) mean fluorescence intensity (MFI) obtained in at least six independent experiments. Values are means plus standard error of the means (SEM) (error bars). Statistical significance was tested using an unpaired t test (*, P < 0.05; **, P < 0.01, ****, P < 0.0001; ns, nonsignificant).