FIG 3.

Treatment with type I IFN or IL-27 enhances Env levels on the surface of HIV-1-infected cells through BST-2 upregulation. Primary CD4⁺ T cells were mock infected or infected with the transmitted/founder virus CH58 (CH58TF) and either treated for 24 h with type I IFN (IFN-α and IFN-β) or IL-27 or not treated. Forty-eight hours postinfection, cells were stained with anti-BST-2 Ab (A) or anti-Env Ab 2G12 (B), followed with appropriate secondary Abs. The graphs shown represent the mean fluorescence intensities obtained for at least eight independent experiments. Statistical significance was tested using a paired t test or Wilcoxon matched-pair signed-rank test based on statistical normality (**, P < 0.01; ***, P < 0.001; ****, P < 0.0001).