FIG 4.

Treatment with type I IFN or IL-27 enhances recognition of HIV-1-infected cells by sera from HIV-1-infected individuals in the presence of CD4mc. Primary CD4⁺ T cells were mock infected or infected with the transmitted/founder virus HIV-1 CH58 (CH58 T/F) and either treated for 24 h with type I IFN (IFN-α and IFN-β) or IL-27 or not treated. Forty-eight hours postinfection, cells were stained with sera from 10 HIV-1-infected individuals in the presence of the CD4mc BNM-III-170 (50 μM) or an equivalent volume of DMSO, followed with appropriate secondary Abs. (A) Histograms depicting representative staining; (B) mean fluorescence intensities (MFI) obtained with all the different sera. Values are means ± standard error of the means (SEM) (error bars). Statistical significance was tested using a paired t test or Wilcoxon matched-pair signed-rank test based on statistical normality (*, P < 0.05; **, P < 0.01, ***, P < 0.001; ****, P < 0.0001; ns, nonsignificant).