TABLE 1.
Steady-state kinetic properties of N9 NAs
| Substrate | NA protein | Mean ± SE Km (μM) | Mean ± SE kcat (s−1) | kcat/Km (μM−1 s−1) |
|---|---|---|---|---|
| MUNANA | Hb+ | 137.9 ± 12.8 | 90.1 ± 4.7 | 0.654 |
| MUNANA | Hb− | 134.2 ± 12.9 | 88.6 ± 4.7 | 0.660 |
| 6SLN | Hb+ | 4,179 ± 615 | 2.2 ± 0.2 | 0.000537 |
| 3SLN | Hb+ | 810 ± 100 | 159.5 ± 8.5 | 0.197 |
| 6SLN | X-31a | 8,070 ± 615 | 18.5 ± 0.9 | 0.00229 |
| 3SLN | X-31a | 562.3 ± 20 | 97.5 ± 1.5 | 0.173 |
| Fetuin | Hb+ | 197.5 ± 37.3 | 71.6 ± 8.3 | 0.363 |
| Fetuin | Hb− | 346.4 ± 95.6 | 73.0 ± 14.5 | 0.211 |
a
The values were determined in a previous study (16).