Table 2.
Differentiation characteristics of strain MUSC 93JT and type strains of phylogenetically closely related species of the genus Streptomyces.
Characteristic | 1 | 2 | 3 | 4 |
---|---|---|---|---|
MORPHOLOGY (ON ISP 2): | ||||
Color of aerial mycelium | Light yellow | Pale greenish yellow | Yellowish white | Light yellow |
Color of substrate mycelium | Vivid yellow | Vivid greenish yellow | Yellowish gray | Brilliant Greenish yellow |
GROWTH AT | ||||
28°C | + | (+) | (+) | + |
36°C | (+) | + | + | + |
pH 6 | + | (+) | (+) | (+) |
2% NaCl | + | (+) | + | (+) |
Catalase | + | + | + | + |
Hemolytic | + | − | − | − |
HYDROLYSIS OF | ||||
Casein (protease) | + | − | − | + |
Tributyrin (lipase) | + | − | + | + |
Starch (amylolytic) | + | + | + | + |
Carboxymethylcellulose (cellulase) | − | + | + | + |
Xylan (xylanase) | − | − | + | − |
CARBON SOURCE UTILIZATION | ||||
D-maltose | − | + | + | + |
Sucrose | − | + | + | + |
D-turanose | − | + | + | + |
D-raffinose | + | − | + | − |
α-D-lactose | − | + | + | + |
β-methyl-D-glucoside | − | + | − | + |
D-salicin | − | + | − | + |
N-acetyl-D-glucosamine | − | + | + | + |
D-fructose | − | + | + | + |
L-rhamnose | − | + | − | + |
D-sorbitol | − | + | + | + |
myo-inositol | − | + | + | + |
Pectin | − | + | + | + |
Methyl pyruvate | − | + | + | + |
D-lactic acid methyl ester | − | + | + | + |
Citric acid | − | + | + | + |
α-keto-glutaric acid | − | + | + | + |
D-malic acid | − | + | + | + |
L-malic acid | − | + | + | + |
Bromo-succinic acid | − | + | + | + |
Propionic acid | − | + | + | + |
Acetic acid | − | + | + | + |
Formic acid | − | + | + | + |
Strains: 1, Streptomyces colonosanans MUSC 93JT; 2, Streptomyces malachitofuscus JCM 4493T; 3, Streptomyces misionensis NBRC 13063T; 4, Streptomyces phaeoluteichromatogenes DSM 41898T. All data were obtained concurrently in this study. +, Positive; -, negative; (+), weak.
All strains are positive for utilization of Dextrin, D-trehalose, D-cellobiose, Gentiobiose, α-D-glucose, D-mannose, D-galactose, glycerol, D-glucose-6-PO4, D-fructose-6-PO4, Gelatin, D-galacturonic acid, L-galactonic acid lactone, D-gluconic acid, D-glucuronic acid, Glucuronamide, p-hydroxy-phenylacetic acid, L-lactic acid, Tween 40, γ-amino-butyric acid, α-hydroxy-butyric acid, β-hydroxy-D,L-butyric acid, α-keto-butyric acid, and acetoacetic acid. All strains are negative for assimilation of 3-methyl glucose.