Table 1.

Growth measurements and lipid productivity of Nannochloropsis salina operated at different nitrogen concentrations and temperatures

	Cell count	Optical density	Dry weight of cells	Lipid productivity
	(×106/ml)	(OD680)	(g L−1)	(g L−1 day−1)
Control	9.93 ± 0.25	0.77 ± 0.04	0.61 ± 0.03	0.23 ± 0.0
75	9.07 ± 0.45a*	0.71 ± 0.03a*	0.57 ± 0.06	0.24 ± 0.0a*
50	8.23 ± 0.45a*b*	0.65 ± 0.02a*b*	0.53 ± 0.05a*	0.30 ± 0.0a*b*
25	7.86 ± 0.45a*b*	0.63 ± 0.02a*b*	0.48 ± 0.02a*b*	0.37 ± 0.0a*b*c*
Temperature°C
15	6.87 ± 0.55	0.53 ± 0.03	0.42 ± 0.05	0.31 ± 0.0
20	7.57 ± 0.35a	0.59 ± 0.02a	0.45 ± 0.04	0.35 ± 0.01a
25	8.23 ± 0.25ab	0.66 ± 0.01ab	0.50 ± 0.04a	0.37 ± 0.01ab
30	8.67 ± 0.06ab	0.71 ± 0.03abc	0.53 ± 0.02ab	0.43 ± 0.0abc
35	8.57 ± 0.25ab	0.72 ± 0.02abc	0.51 ± 0.02a	0.41 ± 0.01abcd

Mean ± SE (n = 3) followed by different letters indicate significant difference at P ≤ 0.05, according to one-way analysis of variance (ANOVA) and the LSD (least significant difference) test (a*: significant with control at ≤0.05, b*: significant with 75% N at ≤0.05, c*: significant with 50% N at ≤0.05, a: significant with 15°C at ≤0.05, b: significant with 20°C at ≤0.05, c: significant with 25°C at ≤0.05, d: significant with 30°C at ≤0.05).