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. 2017 May 15;18:43. doi: 10.1186/s12863-017-0510-x

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Sequencing of homozygous ewes carriers and non-carriers of the FecX ^Bar allele. a Sanger sequencing of the end of first exon of the ovine BMP15 gene. Nucleotide numbering and amino-acid translation are relative to the ATG start site. The FecX ^Bar variant haplotype (Bar/Bar) is indicated within green boxes and compared to wild-type haplotype (+/+). b Protein alignment between reference ovine BMP15 and Barbarine mutated BMP15^A101CfsX113 translated from homozygous FecX ^Bar variant. The 112 alternative amino-acids generated by the frameshift (fs) are highlighted in green. Views were obtained from CLC MainWorkbench 7.6.4 (Qiagen Aarhus)