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. 2017 May 15;18:43. doi: 10.1186/s12863-017-0510-x

Table 1.

Primers used for long-range PCR, sequencing and genotyping of ovine BMP15 and GDF9 genes

Gene Primer sequence (5′→3′) Location Method
BMP15 TTCCTTGCCCTATCCTTTGTG exon 1 long-range PCR/seq.
BMP15 GAGGCCTTGCTACACTAGCC exon 1 genotypingFecX +
BMP15 TGAGAGGCCTTGGCTACACA exon 1 genotypingFecX Bar
BMP15 ACTTTTCTTCCCCATTTTTCTGC exon 1 sequencing
BMP15 CGCTTTGCTCTTGTTCCCTC exon 2 sequencing
BMP15 GGCACTTCATCATTGGACACT exon 2 sequencing
BMP15 GGCAATCATACCCTCATACTCC exon 2 sequencing
BMP15 TCTTCACCCCAAACCGTCTA exon 2 long-range PCR/seq.
GDF9 GAAGACTGGTATGGGGAAATG exon 1 long-range PCR/seq.
GDF9 CCAATCTGCTCCTACACACCT exon 1 sequencing
GDF9 TGGCATTACTGTTGGATTGTTTT exon 2 sequencing
GDF9 TGAACGACACAAGTGCTCAGG exon 2 sequencing
GDF9 GATAGCCCTCTCTTCTGGTCA exon 2 sequencing
GDF9 GCTCCTCCTTACACAACACACAG exon 2 sequencing
GDF9 GTGTTCTTACCTGGCAAACC exon 2 long-range PCR/seq.