Fig. 3.

EAR16 but not EAR18 shows selectivity for GluN2B over GluN2A. a, b EAR16-mediated inhibition of NMDA-evoked inward currents on HEK-293 cells expressing GluN1A–GluN2B subunits (a) or expressing GluN1A–GluN2A subunits (b). d, e EAR18-mediated inhibition of NMDA-evoked inward currents on HEK-293 cells expressing GluN1A–GluN2B (d) or in cells GluN1A–GluN2A subunits (e). c, f The areas of the NMDA-evoked inward currents were normalized to that measured prior to the addition of EAR16 (c) or EAR18 (f) (−1: maximal inward current; 0: no current). No significant difference was found between “control-NMDA” and “wash-NMDA”. Significant different compared to “control-NMDA” (left symbol) or when compared to “wash-NMDA” (right symbol) #p < 0.0001, +p < 0.001, @p < 0.02, two-way ANOVA with Sidak’s multiple comparison post-test; n (number of cells) EAR16, n = 4 for GluN1A–GluN2B, and n = 4 for GluN1A–GluN2A; EAR18 n = 3 for GluN1–GluN2B (washout n = 2) and n = 4 for GluN1A–GluN2A