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. 2017 May 15;18:379. doi: 10.1186/s12864-017-3746-y

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Venn Diagram generated using combineOfftargets to depict the overlaps of off-target sites between three different nuclease variants. Example of the output from the combineOfftargets function (Example 6) comparing the overlap in GUIDE-seq identified off-target sites for wild-type Cas9, Split-Cas9 (dual NLS) [51], and the highly specific SpCas9^MT3-ZFP [25] programmed with a sgRNA recognizing VEGFA site 2 (TS2) [11]