Fig. 4.

Gross images of alkaline phosphatase staining in the upper and lower respiratory tract of mice following administration of an AAV vector expressing hPLAP using the intubation technique. AAV vector (1x10¹¹ vg) was administered by endotracheal intubation to 7-week-old C57BL/6 mice and tissues evaluated for hPLAP expression after three weeks. Injection via intubation resulted in somewhat variable hPLAP staining of the upper and lower respiratory tract, including the distal aspect of the lung. Staining in the nasal epithelium and trachea was not as intense as with the intranasal methods. Note that some lung lobes were highly transduced with vector distribution throughout the entire lung lobe (see A3, A5 and D1). Letters a-d represent individual mice and numbers one through five indicate the accessory lobe (1), the left lobe (2), the right cranial lobe (3), the right caudal lobe (4) and the right middle lobe (5)