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. 2017 May 15;19:91. doi: 10.1186/s13075-017-1284-2

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Effect of Emab on cell proliferation. Tonsillar CD10^–CD27^– B cells were negatively selected using magnetic bead cell enrichment and then stimulated with TLR7 agonist R848 and/or F(ab′)2 anti-human IgM with or without Emab or a human IgG control. Cells were labeled with CFSE and cultured for 3 days with different stimuli, and levels of cell proliferation were measured based on CFSE dilution. Gates depict CFSE^lo (proliferating) cells. a Flow cytometry data from one representative experiment. b Cumulative data from five independent experiments using CD10^–CD27^– enriched B cells from different donors. *p < 0.05 by two-way ANOVA analysis with Bonferroni post tests. Emab epratuzumab