Fig. 4.

Emab selectively inhibits PRDM1 mRNA expression in CD10^–CD27^– B cells. a Network of transcription factors involved in B-cell differentiation into Ab-producing cells. b–d Tonsillar CD10^–CD27^– B cells were negatively selected using magnetic bead cell separation and then stimulated with R848 (TLR7 agonist) and/or F(ab′)2 anti-human IgM with or without Emab or a human IgG control. RNA was isolated 12 hours after stimulation and expression of different genes was quantified by RT-PCR. *p < 0.05, **p < 0.01 as determined by two-way ANOVA with Bonferroni post test. Emab epratuzumab