Fig. 7.

Emab inhibits the generation of Blimp1⁺ cells. Sorted CD19⁺CD10^–CD27^–IgD^– tonsillar B cells were left untreated or stimulated with R848, anti-human F(ab′)₂ IgM or a combination of R848 plus anti-IgM in the presence of Emab or isotype control Ab. a Representative flow cytometry data, showing intracellular Blimp1 expression within CD10^–CD27^–IgD^– B cells after 3 days of cell culture in the presence of Emab (black line) or an isotype-matched control Ab (gray line). PE-conjugated rat IgG2a was used as a staining control (dotted tinted line). b Representative flow cytometry data showing CD20 and intracellular Blimp1 expression within CD10^–CD27^–IgD^– cells after 3 days of cell culture. Gates depict frequencies of CD20^loBlimp1⁺ cells. c Summary results from three independent experiments showing the percentage of CD20^loBlimp1⁺ from total live cells in culture after cell stimulation in the presence or absence of Emab. **p < 0.01, as determined by unpaired Student’s t test. Emab epratuzumab