Table 2.
Primers used for qPCR and multiplex HRM-qPCR analysis
| Target gene | Sequence (5′--3′) (name) | Size (bp) | TA(°C)a | Reference |
|---|---|---|---|---|
| K99 fimbriae (fan A) | CACTTGAGGGTATATGCGATCTT (K99 F) | 92 | 62 | This study |
| GACCTCAGTCACAGCAACTATAC (K99 R) | ||||
| F41 fimbriae Sub-unit A | GACCTCAGTCACAGCAACTATAC (F41 F) | 110 | 62 | This study |
| CGACCCGCAACATCCTTATT (F41 R) | ||||
| F18 fimbriae (Fed A) | GGAGGTTAAGGCGTCGAATAG (F18 F) | 90 | 62 | This study |
| CCACCTTTCAGTTGAGCAGTA (F18 R) | ||||
| F6 fimbriae (Fas A) | GTTCCAGCCTCCAATGATACT (F6 F) | 128 | 62 | This study |
| GAAAGAGCTAATCCGCCATTTG (F6 R) | ||||
| K88 fimbriae (fae G) | GCACATGCCTGGATGACTGGTG (K88 F) | 439 | 63 | [54, 55] |
| CGTCCGCAGAAGTAACCCCACCT (K88 R) | ||||
| E. coli (Universal stress protein A) | CCGATACGCTGCCAATCAGT (UspA F) | 884 | 66 | [26, 54] |
| ACGCAGACCGTAGGCCAGAT (UspA R) | ||||
| Heat-labile toxin | CCGTGCTGACTCTAGACCCCCA (LT F) | 480 | 68 | [54, 56] |
| CCTGCTAATCTGTAACCATCCTCTGC (LT R) | ||||
| Heat-stable toxins a | ATGAAAAAGCTAATGTTGGC (STa F) | 193 | 65 | [28, 54] |
| TACAACAAAGTTCACAGCAG (STa R) | ||||
| Heat-stable toxins b | TGCCTATGCATCTACACAAT (STb F) | 113 | 60 | [54, 57] |
| CTCCAGCAGTACCATCTCTA (STb R) |
aTA, primer annealing temperature