Table 3.

Melting temperature and calibration parameters for multiplex HRM-qPCR detection of K99, F41, F18, F6 and K88 fimbriae gene

	K99	F41	F18	F6		K88
Tm(°C)	74.7 ± 0.06	76.5 ± 0.12	77.6 ± 0.35	79.5 ± 0.78		80.5 ± 0.15
E a	1.87	1.74	1.7	1.85		1.84
r1 2 b	0.99	0.99	0.99	0.99		0.99
DNA range	1–20%	0.2%–16.7%	1%–100%	0.3%–4.8%	4.8%–60%	4.8%–20%
Slope c	2.96	2.74	2.39	0.48	1.44	1.49
r2 2 d	0.97	0.97	0.98	0.97	0.99	0.97

^aprimer efficiency determined by individual PCR

^br₁ ², correlation coefficient for standard curve determined by individual qPCR

^cSlope for calibration curve correlating the area of the melting peaks area to the proportion of the template DNA

^dr₂ ², correlation coefficient for standard curve correlating the area of the melting peaks to the proportion of the template DNA